Journal
SCIENCE
Volume 297, Issue 5581, Pages 612-615Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1072309
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Funding
- NIAID NIH HHS [AI19883] Funding Source: Medline
- NIGMS NIH HHS [GM59216] Funding Source: Medline
- CGH CDC HHS [GH39066] Funding Source: Medline
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Nucleation of branched actin laments by the Arp2/3 complex is a conserved process in eukaryotic cells, yet the source of unbranched actin laments has remained obscure. In yeast, formins stimulate assembly of actin cables independently of Arp2/3. Here, the conserved core of formin homology domains 1 and 2 of Bni1p (Bni1pFH1FH2) was found to nucleate unbranched actin laments in vitro. Bni1pFH2 provided the minimal region sufficient for nucleation. Unique among actin nucleators, Bni1pFH1FH2 remained associated with the growing barbed ends of laments. This combination of properties suggests a direct role for formins in regulating nucleation and polarization of unbranched filamentous actin structures.
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