4.7 Article

High-throughput assay of oxygen radical absorbance capacity (ORAC) using a multichannel liquid handling system coupled with a microplate flourescence reader in 96-well format

Journal

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 50, Issue 16, Pages 4437-4444

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/jf0201529

Keywords

ORAC; antioxidant activity; free radical; plate reader; high throughput

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The oxygen radical absorbance capacity (ORAC) assay has-been widely accepted as a standard tool to measure the, antioxidant activity in the nutraceutical, pharmaceutical, and food industries. However, the ORAC assay has been criticized for a lack of accessibility due to the unavailability of the COBAS FARA II analyzer, an instrument discontinued by the manufacturer. In addition, the manual sample preparation is time-consuming and labor-intensive. The objective of this study was to develop a high-throughput instrument platform that can fully automate the ORAC assay procedure. The new instrument platform consists of a robotic eight-channel liquid handling system and a microplate fluorescence reader. By using the high-throughput platform, the efficiency of the assay is improved with at least a 10-fold increase in sample throughput over the current procedure. The mean of intra- and interday CVs was less than or equal to15%, and the limit of detection and limit of quantitation were 5 and 6.25 muM, respectively.

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