Journal
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
Volume 48, Issue 2, Pages 70-76Publisher
BLACKWELL MUNKSGAARD
DOI: 10.1034/j.1600-0897.2002.01071.x
Keywords
cytokine; trophoblast; vascular endothelial growth factor
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PROBLEM: The mechanism through which vascular endothelial growth factor (VEGF) regulation occurs at the feto-maternal interface is poorly understood. The aim of this study was to investigate the effects of various cytokines on VEGF expression and secretion by trophoblast cells. METHOD OF STUDY: We investigated the effects of cytokines on VEGF expression in human first trimester trophoblast cell line by analyzing VEGF messenger RNA (mRNA) by reverse transcription-polymerase chain reaction and VEGF protein secretion by enzyme linked immunosorbent assay. RESULTS: The trophoblast cells expressed VEGF mRNA constitutively and the main subtypes were identified as VEGF121 and VEGF165. When cultured in the presence of interferon (IFN)-gamma, interleukin (IL)-1beta, tumor necrosis factor (TNF)-alpha IL-2. or IL-10. VEGF mRNA expression was found to be significantly increased by IL-1beta, IFN-gamma and TNF-alpha but to be unaffected by IL-2 and IL-10. Moreover, VEGF secretion was most significantly increased by IFN-gamma treatment. CONCLUSION: These results suggest that IL-1beta. IFN-gamma and TNF-alpha may regulate the production of VEGF in early gestational trophoblasts.
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