Journal
STRUCTURE
Volume 10, Issue 8, Pages 1117-1125Publisher
CELL PRESS
DOI: 10.1016/S0969-2126(02)00813-4
Keywords
transcription regulation; transcription initiation; Rpb4/Rpb7; cryo-electron microscopy; RNA polymerase conformation
Funding
- NIGMS NIH HHS [GM60607] Funding Source: Medline
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An 18 Angstrom resolution structure of the 12-subunit yeast RNA polymerase II (RNAPII) calculated from electron microscope images of single particles preserved in amorphous ice reveals the conformation of the enzyme in solution. The Rpb4/Rpb7 polymerase subunit complex was localized and found to be ideally positioned to determine the path of the nascent RNA transcript. The RNAPII structure suggests a revised mode of interaction with promoter DNA and demonstrates that regulation of RNAPII must involve structural changes that render the enzyme competent for initiation.
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