Journal
JOURNAL OF VIROLOGICAL METHODS
Volume 105, Issue 1, Pages 171-179Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0166-0934(02)00101-5
Keywords
real-time PCR; dual-labelled fluorogenic probes; EIAV; quantitation of viral load
Funding
- NCI NIH HHS [R01CA49296] Funding Source: Medline
- PHS HHS [R01A12850] Funding Source: Medline
Ask authors/readers for more resources
A single-tube reverse transcriptase-polymerase chain reaction (RT-PCR) using a fluorogenic real-time PCR detection method is described for the quantitation of equine infectious anemia virus (EIAV) RNA in the plasma of equids. To compensate for variations inherent in sample preparation a multiplex real-time RT-PCR system was developed that permitted the simultaneous calculation of the nucleic acid recovery rate along with the copy number of viral RNA molecules. Detection of EIAV RNA was linear from 10(9) to 10(1) molecules with intra- and inter-assay variability of less than 1% at 10(8), 10(6), 10(4) and 10(2) molecules. (C) 2002 Elsevier Science B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available