Journal
BIOMOLECULAR ENGINEERING
Volume 19, Issue 2-6, Pages 281-285Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S1389-0344(02)00038-2
Keywords
plasmin; fibrinogen; graphite and glassy carbon; contact angles; Langmuir-Blodgett
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The amidolytic activity of plasmin (Pln) that spontaneously adsorbs from solutions to modified-graphite (GR) and glassy carbon (GL) surfaces was studied in the 10-45 degreesC temperature range in the presence of a chromogenic substrate. Surfaces were modified with a coating of fibrinogen, either electrochemically oxidized or not. The effect of an additional modification via deposition of Langmuir-Blodgett films of behenic acid (BA-LB) onto the former surfaces, thus leading to either hydrophobic or hydrophilic surfaces according to the number of deposed layers, was examined. In all cases results showed the occurrence of a first order transition which strongly and transiently increases the surface activity of Pln. At BA-LB surfaces, the most prominent change compared with other coatings was a significant enhancement of the critical temperature Tc that characterizes the beginning of the transition. When fibrinogen was present in the solution, the transition was no longer observable up to 37 degreesC as shown by the linear kinetics exhibited by surfaces bearing oxidized fibrinogen. (C) 2002 Elsevier Science B.V. All rights reserved.
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