Journal
CURRENT OPINION IN BIOTECHNOLOGY
Volume 13, Issue 4, Pages 321-328Publisher
ELSEVIER SCI LTD
DOI: 10.1016/S0958-1669(02)00333-6
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To date, the most widely used technology for conducting proteomic studies has been two-dimensional gel electrophoresis (2DGE), but this approach does have drawbacks. Isotope-coded affinity tagging (ICAT) is starting to challenge 2DGE as a new proteomic tool for the analysis of proteins in complex biological specimens. An appraisal of these two methodologies reveals that neither ICAT nor 2DGE provide comprehensive coverage on a proteome-wide scale.
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