Journal
TRAFFIC
Volume 3, Issue 8, Pages 590-600Publisher
BLACKWELL MUNKSGAARD
DOI: 10.1034/j.1600-0854.2002.30808.x
Keywords
Alzheimer's; AP-2 adaptor; clathrin; endocytosis; membrane traffic
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Funding
- NIGMS NIH HHS [GM36548] Funding Source: Medline
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The endocytic sorting signal on the low-density lipoprotein receptor for clathrin-mediated internalization is the sequence FDNPVY in the receptor's cytosolic tail. We have used a combination of surface plasmon resonance and crosslinking with a photoactivated peptide probe to demonstrate the interaction between FDNPVY-containing peptides and the mu2 chain of purified AP-2 clathrin adaptors (the complexes responsible for plasma membrane sorting). We show that recognition of the FDNPVY signal is mediated by a binding site in the mu2-subunit that is distinct from the site for the more general YppO sorting signal, another tyrosine-based sequence also recognized by mu2-adaptin. These results suggest the possibility that low-density lipoprotein receptor uptake may be modulated specifically and independently of other proteins in the clathrin pathway.
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