Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 99, Issue 16, Pages 10276-10281Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.102327699
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Funding
- NCI NIH HHS [R24 CA088261, R24 CA-88261] Funding Source: Medline
- NIDA NIH HHS [DA-00376, P30 DA013429, DA-06650, DA-14230, P30 DA-13429, DA-11130, R01 DA006650, R01 DA014230] Funding Source: Medline
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The chemokines use G protein-coupled receptors to regulate the migratory and proadhesive responses of leukocytes. Based on observations that G protein-coupled receptors undergo heterologous desensitization, we have examined the ability of chemokines to also influence the perception of pain by cross-desensitizing oploid G protein-coupled receptors function in vitro and in vivo. We find that the chemotactic activities of both mu- and delta-oploid receptors are desensitized following activation of the chemokine receptors CCR5, CCR2, CCR7, and CXCR4 but not of the CXCR1 or CXCR2 receptors. Furthermore, we also find that pretreatment with RAN-TES/CCL5, the ligand for CCR1, and CCR5 or SDF-1alpha/CXCL12, the ligand for CXCR4, followed by oploid administration into the periaqueductal gray matter of the brain results in an increased rat tail flick response to a painful stimulus. Because chemokine administration into the periaqueductal gray matter inhibits opioid-induced analgesia, we propose that the activation of proinflammatory chemokine receptors down-regulates the analgesic functions of oploid receptors, and this enhances the perception of pain at inflammatory sites.
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