Journal
CURRENT BIOLOGY
Volume 12, Issue 15, Pages 1317-1319Publisher
CELL PRESS
DOI: 10.1016/S0960-9822(02)01041-2
Keywords
-
Categories
Funding
- NIGMS NIH HHS [GM37706, R01 GM037706] Funding Source: Medline
- Wellcome Trust [054523] Funding Source: Medline
Ask authors/readers for more resources
RNA interference (RNAi) is a broadly used reverse genetics method in C. elegans [1]. Unfortunately, RNAi does not inhibit all genes [2, 3]. We show that loss of function of a putative RNA-directed RNA polymerase (RdRP) of C. elegans, RRF-3, results in a substantial enhancement of sensitivity to RNAi in diverse tissues. This is particularly striking in the nervous system; neurons that are generally refractory to RNAi in a wildtype genetic background can respond effectively to interference in an rrf-3 mutant background. These data provide the first indication of physiological negative modulation of the RNAi response and implicate an RdRP-related factor in this effect. The rrf-3 strain can be useful to study genes that, in wild-type, do not show a phenotype after RNAi, and it is probably the strain of choice for genome-wide RNAi screens.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available