Journal
FEBS LETTERS
Volume 525, Issue 1-3, Pages 33-38Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(02)03061-2
Keywords
lipid bilayer; membrane insertion; oligomerization; protein-lipid interaction; thermo-stability
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Recently we observed in an in vitro system that newly synthesized KcsA assembles efficiently into a tetramer in lipid vesicles [van Dalen et A. (2002) FEBS Lett. 511, 51-58]. Here we used this system to get insight into the importance of the lipid composition for KcsA membrane association and tetramerization and we compared this to the lipid dependency of the thermo-stability of the KcsA tetramer. It was found that a large amount of phosphatidylethanolamine (> 40 mol%) and a lower amount of phosphatidylglycerol (similar to 20-30 mol%) were optimal for efficient KcsA membrane association and tetramerization. Strikingly, vesicles of the abundant and commonly used membrane lipid phosphatidylcholine did not support assembly, further demonstrating the importance of membrane lipid composition for KcsA assembly. The in vitro assembled KcsA tetramer showed similar thermo-stability in biological and pure lipid membranes, demonstrating that both tetramers are alike. In addition, we show that solubilization of the membrane with detergent reduces the thermo-stability of the tetramer. The highest KcsA tetramer stability was observed in intact bilayers in the presence of anionic lipids. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
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