Journal
EXPERIMENTAL PHYSIOLOGY
Volume 87, Issue 5, Pages 575-583Publisher
CAMBRIDGE UNIV PRESS
DOI: 10.1113/eph8702436
Keywords
-
Categories
Funding
- NHLBI NIH HHS [HL63903] Funding Source: Medline
Ask authors/readers for more resources
We have examined the influence of innervation on the expression of different isoforms of sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) in regenerating rat Slow twitch muscle. The process of degeneration/regeneration was induced by injection of bupivacaine into rat soleus muscle under four different conditions: (1) in the presence of intact motor nerves, (2) after surgical denervation, (3) with nerve impulse conduction blocked by the Ne-channel blocker tetrodotoxin (TTX), and (4) with the axoplasmic flow blocked by vinblastine. Expression of SERCA isoforms was visualized by immunohistochemical and Western blot analysis. In regenerating innervated muscle, SERCA1, the isoform normally expressed in fast twitch fibres, was present after 5 days and was then progressively replaced by SERCA2, the isoform typical of slow twitch fibres. The maximum Ca2+ uptake, measured in single skinned fibres regenerating for 10-21 days, was similar to that of slow adult fibres and significantly lower than that of fast adult fibres. Denervation or TTX treatment prevented the expression of the SERCA2 isoform. Conversely, vinblastine did not affect the expression of SERCA isoforms. These data indicate that nerve impulses play a determinant role in the expression of the SERCA2 isoform.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available