Journal
JOURNAL OF VIROLOGY
Volume 76, Issue 18, Pages 9551-9555Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.76.18.9551-9555.2002
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An endothelial cell-tropic and leukotropic human cytomegalovirus (HCMV) clinical isolate was cloned as a fusion-inducing factor X-bacterial artificial chromosome in Escherichia coli, and the ribonucleotide reductase homolog UL45 was deleted. Reconstituted virus RVFIX and RVDeltaUL45 grew equally well in human fibroblasts and human endothellial cells. Thus, UL45 is dispensable for growth of HCMV in both cell types.
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