Journal
EUROPEAN JOURNAL OF BIOCHEMISTRY
Volume 269, Issue 18, Pages 4446-4457Publisher
WILEY
DOI: 10.1046/j.1432-1033.2002.03150.x
Keywords
isoprenoid; 2-C-methyl-D-erythritol 4-phosphate; 1-deoxyxylulose 5-phosphate reductoisomerase; 2-C-methyl-D-erythrose 4-phosphate
Categories
Ask authors/readers for more resources
The 1-deoxyxylulose 5-phosphate reductoisomerase (DXR, EC 1.1.1.267) catalyzes the conversion of 1-deoxy-d-xylulose 5-phosphate (DXP) into 2-C -methyl-d-erythritol 4-phosphate (MEP). This transformation is a two-step process involving a rearrangement of DXP into the putative intermediate 2-C -methyl-d-erythrose 4-phosphate followed by a NADPH-dependent reduction of the latter aldehyde. By using [1-(13) C]DXP as a substrate, the rearrangement of DXP into [5-(13) C]2-C -methyl-d-erythrose 4-phosphate was shown to be NADPH dependent, although it does not involve areduction step. The putative aldehyde intermediate, obtained by chemical synthesis, was converted into MEP by the DXR in the presence of NADPH and into DXP in the presence of NADP(+) , indicating the reversibility of the reaction catalyzed by the DXR. This reversibility was confirmed by the conversion of MEP into DXP in the presence of NADP(+) . The equilibrium was, however, largely displaced in favour of the formation of MEP. The reduction step required the presence of a divalent cation such as Mg2+ or Mn2+ .
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available