Journal
INFECTION AND IMMUNITY
Volume 70, Issue 9, Pages 5290-5294Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/IAI.70.9.5290-5294.2002
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Using a transposon mutagenesis approach, we have identified a mutant of Burkholderia pseudomallei that is auxotrophic for branched chain amino acids. The transposon was shown to have interrupted the ilvI gene encoding the large subunit of the acetolactate synthase enzyme. Compared to the wild (type, this mutant was significantly attenuated in a marine model of disease. Mice inoculated intraperitoneally with the auxotrophic mutant, 35 days prior to challenge, were protected against a challenge dose of 6,000 median lethal doses of wild-type B. pseudomallei.
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