Journal
NATURE CELL BIOLOGY
Volume 4, Issue 9, Pages 691-698Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/ncb837
Keywords
-
Categories
Funding
- NIDDK NIH HHS [DK44239] Funding Source: Medline
- NIMH NIH HHS [MH60600] Funding Source: Medline
Ask authors/readers for more resources
As a final step in endocytosis, clathrin-coated pits must separate from the plasma membrane and move into the cytosol as a coated vesicle. Because these events involve minute movements that conventional light microscopy cannot resolve, they have not been observed directly and their dynamics remain unexplored. Here, we used evanescent field (EF) microscopy to observe single clathrin-coated pits or vesicles as they draw inwards from the plasma membrane and finally lose their coats. This inward movement occurred immediately after a brief burst of dynamin recruitment and was accompanied by transient actin assembly. Therefore, dynamin may provide the trigger and actin may provide the force for movement into the cytosol.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available