Journal
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
Volume 283, Issue 3, Pages H1134-H1141Publisher
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpheart.00065.2002
Keywords
infarction; myocardial necrosis; myocardial platelet accumulation; P-selectin
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Platelets (Plt) accumulate in reperfused myocardium but their effect on myocardial necrosis has not been established. We tested the hypothesis that the effect of Plt depends on their activation status. Pig Plt were obtained before 48 min of coronary occlusion (pre-CO-Plt), 10 min after reperfusion (R-Plt), or after a 60-min sham operation (sham-Plt). Plt were infused into isolated rat hearts (n=124) and subsequently submitted to 60 min of ischemia and 60 min of reperfusion. P-selectin expression was higher (P=0.02) in R-Plt than in pre-CO-Plt or sham-Plt. Lactate dehydrogenase (LDH) release during reperfusion was similar in hearts receiving pre-CO-Plt, sham-Plt, or no Plt, but R-Plt increased LDH release by 60% (P=0.004). Activation of pre-CO-Plt with thrombin increased P-selectin expression and LDH release (P<0.001), and these results were unaffected by tirofiban. There was a close correlation between P-selectin expression and LDH release (r=0.84; P<0.001), and myocardial Plt accumulation (r=0.85; P<0.001). We conclude that the deleterious effect of Plt on reperfused myocardium depends on their activation status as represented by P-selectin expression, which is enhanced by ischemia-reperfusion.
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