Journal
PLANTA
Volume 215, Issue 5, Pages 708-715Publisher
SPRINGER
DOI: 10.1007/s00425-002-0816-3
Keywords
cyclophilin; nitrite; nitric oxide; peroxynitrite; 14-3-3 protein; tyrosine nitration
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An antisense nitrite reductase (NiR, EC 1.7.7.1) tobacco (Nicotiana tabacum L.) transformant (clone 271) was used to gain insight into a possible correlation between nitrate reductase (NR, EC 1.6.6.1)-dependent nitrite accumulation and nitric oxide (NO) production, and to assess the regulation of signal transduction in response to stress conditions. Nitrite concentrations of clone 271 leaves were 10-fold, and NO emission rates were 100-fold higher than in wild type leaves. Increased protein tyrosine nitration in clone 271 suggests that high NO production resulted in increased peroxynitrite (ONOO-) formation. Tyrosine nitration was also observed in vitro by adding peroxynitrite to leaf extracts. As in mammalian cells, NO and derivatives also increased synthesis of proteins like 14-3-3 and cyclophilins, which are both involved in regulation of activity and stability of enzymes.
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