Detection of tumor necrosis factor α in normal and inflamed human dental pulps

Background. The aim of this study was to determine concentrations of tumor necrosis factor-alpha (TNF-alpha) in normal, painful, and asymptomatic human dental pulps. Methods. Pulps were obtained from three groups of teeth, including healthy teeth, asymptomatic teeth with caries and/or large restoration, and symptomatic teeth with clinical diagnosis of irreversible pulpitis. Pulpal tissues were collected, prepared, and analyzed for TNF-alpha concentration by ELISA technique. Results. Nonparametric Kruskal-Wallis test revealed significant differences between TNF-alpha concentration in normal samples (64.01 +/- 53.12 pg/g) and irreversible symptomatic pulpal tissue (1,962.99 +/- 1,288.75 pg/g), between irreversible symptomatic and asymptomatic (1, 120.09 +/- 649.72 pg/g), and between normal and irreversible asymptomatic pulpal tissue (p = 0.000). Conclusions. TNF-alpha may be an objective marker for determining extent of pulpal inflammation associated with irreversible pulpitis. (C) 2002 IMSS. Published by Elsevier Science Inc.