Journal
JOURNAL OF BIOSCIENCES
Volume 27, Issue 5, Pages 515-520Publisher
INDIAN ACADEMY SCIENCES
DOI: 10.1007/BF02705049
Keywords
Escherichia coli; D-amino acids; L-amino acids; osmolyte; stress
Categories
Ask authors/readers for more resources
Adaptation to high salinity and low or high temperature is essential for bacteria to survive. Accumulation of exogenous osmolytes is one of the ways that helps bacteria to survive under such extracellular stress. We have analysed the capability of various L-amino acids and their D-isomers to act as osmolytes and thus enable Escherichia coli cells to survive under various stress conditions. E. coli cells were grown in the presence or absence of L- and D-proline, alanine, serine and lysine under salt, heat and cold stresses. Of the various amino acids tested, L-proline, closely followed by L-serine turned out to be highly protective against environmental stresses. L-proline provided excellent protection (95%) against salt stress, followed by cold (60%) and heat (40%) stresses. D-amino acids on the other hand, proved to be highly inhibitory under stress conditions. Thus L-amino acids were found to be growth protectants under stress while their D-isomers were inhibitory during stress as well as normal conditions.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available