Journal
HEARING RESEARCH
Volume 171, Issue 1-2, Pages 119-128Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0378-5955(02)00493-8
Keywords
cochlea; vestibular system; ossicles; guinea pig; imaging; microwave
Funding
- NIDCD NIH HHS [1R43 DC 03623-01, 2R44 DC 03623-02] Funding Source: Medline
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Orthogonal-plane fluorescence optical sectioning (OPFOS) microscopy was developed for the purpose of making quantitative measurements of the intact mammalian cochlea and to facilitate 3D reconstructions of complex features. A new version of this imaging apparatus was built with a specimen chamber designed to accommodate samples as large as the intact guinea pig bulla. This method left the cochlear connections with the vestibular system and with the ossicles of the middle car undisturbed, providing views within the cochlea with no breaches of its structural integrity. Since the features within the bulla were not physically touched during the preparation process, the risk of damage was minimized, and were imaged in relatively pristine condition with spatial resolution to 16 mum. A description of the imaging method and specimen preparation procedure is presented, as are images of features from the cochlea, ossicles, and vestibular system. (C) 2002 Elsevier Science B.V. All rights reserved.
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