Journal
ELECTROCHEMISTRY COMMUNICATIONS
Volume 4, Issue 9, Pages 722-726Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/S1388-2481(02)00434-4
Keywords
nanoparticles; DNA hybridization; cadmium sulfide; stripping potentiometry
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We report on the detection of DNA hybridization in connection to cadmium sulfide nanoparticle tracers and electrochemical stripping measurements of the cadmium. A nanoparticle-promoted cadmium precipitation is used to enlarge the nanoparticle tag and amplify the stripping DNA hybridization signal. In addition to measurements of the dissolved cadmium ion c demonstrate solid-state measurements following a 'magnetic' collection of the magnetic-bead/DNA-hybrid/CdS-tracer assembly onto a thick-film electrode transducer. The new protocol combines the amplification features of nanoparticle/polynucleotides assemblies and highly sensitive stripping potentiometric detection of cadmium. with an effective magnetic isolation of the duplex. The low detection limit (100 fmol) is coupled to good reproducibility (RSD = 6%). Prospects for using binary inorganic colloids for multi-target detection are discussed. (C) 2002 Elsevier Science B.V. All rights reserved.
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