Journal
PHYTOCHEMISTRY
Volume 61, Issue 1, Pages 1-6Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0031-9422(02)00144-9
Keywords
kiwi fruit; Actinidia chinensis; Actinidiaceae; thaumatin-like protein; antifungal
Categories
Ask authors/readers for more resources
A single-chain 21 kDa protein exhibiting antifungal activity against Botrytis cinerea and some suppressive effects on Mycosphaerella arachidicola and Coprinus comatus was isolated from kiwi fruits. The protein, designated kiwi fruit thaumatin-like protein, did not inhibit translation in the cell-free rabbit reticulocyte lysate system but inhibited HIV-1 reverse transcriptase. It was purified to apparent homogeneity using a procedure involving saline extraction, (NH4)(2)SO4 precipitation, ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography on SP-Sepharose and gel filtration on Superdex 75, respectively. (C) 2002 Elsevier Science Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available