Journal
EUROPEAN JOURNAL OF CANCER
Volume 38, Issue 13, Pages 1792-1801Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0959-8049(02)00139-9
Keywords
distamycin derivative; telomerase; telomere; apoptosis; melanoma; non-small cell lung carcinoma; osteogenic sarcoma
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In this study, we evaluated the potential of the distamycin derivative MEN 10716 as a telomerase inhibitor. Exposure of human melanoma cell extracts to MEN 10716 induced a dose-dependent inhibition of telomerase activity, with an IC50 of 24 +/- 3 muM. When intact JR8 melanoma cells were chronically exposed to the drug (200 muM every other day for 50 days). a marked inhibition (> 80%) of the enzyme's catalytic activity was consistently observed starting from day 1. At later points in time. MEN 10716 inhibited melanoma cell proliferation and induced apoptosis Cells surviving MEN 10716 exposure were characterised by a higher melanin content and a greater expression of p16(INK4A) protein than control cells. The effects of MEN 10716 were subsequently evaluated in different tumour cell systems. In particular, even in the H460 non-small cell lung cancer cell line, chronic exposure of the cells to the drug (100 muM every other day for 50 days) induced a consistent inhibition (> 85%) of telomerase activity, a reduction of cell proliferation potential, and apoptosis. Conversely, MEN 10716 treatment did not appreciably inhibit cell proliferation in the U2-OS telomerase-negative human osteogenic sarcoma cell line. Interestingly, no variation in the mean telomere length was observed in MEN 10716-treated JR8 melanoma cells, whereas an appreciable increase in the mean telomere length was found in H460 lung cancer cells after drug exposure. Overall, the results of the study indicate that MEN 10716 is a possible telomerase inhibitor and suggest that abrogation of telomerase activity can affect cell proliferation even through pathways that are not dependent oil telomere erosion. (C) 2002 Elsevier Science Ltd. All rights reserved.
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