Polyelectrolyte-stabilized biosensors based on macroporous carbon electrode

In this work, polyelectrolyte-stabilized enzymes are used for the construction of stable biosensors utilizing a simple manufacture procedure. The stabilized enzyme is immobilized by physical adsorption into activated macroporous and conductive carbon material, which serves as the immobilization matrix and the electrochemical transducer. The amount of polyelectrolyte used is optimized with respect to sensor's sensitivity and stability. The optimized biosensors exhibit high operational stability and good sensor-to-sensor reproducibility. (C) 2002 Elsevier Science B.V. All rights reserved.