Journal
GENES & DEVELOPMENT
Volume 16, Issue 18, Pages 2379-2389Publisher
COLD SPRING HARBOR LAB PRESS
DOI: 10.1101/gad.1020702
Keywords
Drosophila melanogaster; STAT92E; alternative promoters; differential splicing
Categories
Funding
- NIAID NIH HHS [AI32489, R37 AI034420, AI34420, R01 AI032489] Funding Source: Medline
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Previously unrecognized mRNAs originating from a dual promoter at the stat92E locus are described. One of these encodes a truncated protein, DeltaNSTAT92E, that lacks the N-terminal 133 amino acids. Antibodies detect both the full-length and truncated molecules early in embryogenesis (1-5 h), and mRNA detection by specific RT-PCR reactions accords with the protein distribution. Given that the N termini of mammalian STATs are known to have positive functions in transcriptional activation, we explored the role of DeltaNSTAT92E early in embryogenesis. By increasing the DeltaNSTAT92E-to-STAT92E ratio in overexpression and RNAi experiments, we observe phenotypes compatible with suppression of wild-type STAT92E activity. We therefore conclude that the short form of STAT92E is a naturally occurring dominant-negative product that can be added to the 0 Growing list of negative regulators of STAT activity.
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