Journal
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
Volume 405, Issue 2, Pages 147-153Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/S0003-9861(02)00341-7
Keywords
proteasome; immunoproteasome; interferon gamma; RT-PCR; ocular lens; mouse; transgenic; alpha TN4-1 cells
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Funding
- NEI NIH HHS [EY02299] Funding Source: Medline
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Interferon gamma (IFNgamma) induces the expression of three catalytic subunits of the 208 proteasome that can replace their constitutive homologues to form the immunoproteasome, named to reflect its antigen presentation function. However, immunoproteasome levels and their modulation in nonimmune tissues remain unknown. A disrupted lens differentiation program observed in transgenic mice that constitutively express IFN-gamma in the immune-privileged lens tissue suggests a role for this cytokine in differentiation. We have developed a competitive RT-PCR assay that demonstrates substantially increased levels of immuno subunits and unchanged levels of constitutive subunits in transgenic compared to wild-type lenses. Similar results were observed with IFNgamma treated alphaTN4-1 lens epithelial cells. A comparison of these subunits in different immune and nonimmune mouse tissues revealed unique expression patterns. The presence of immuno subunits in nonimmune tissues such as lens suggests that the immunoproteasome may also have nonimmune functions, such as that in lens differentiation. Published by Elsevier Science (USA).
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