Journal
ANALYTICA CHIMICA ACTA
Volume 469, Issue 1, Pages 149-154Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0003-2670(01)01537-9
Keywords
breast cancer detection; BRCA; SERS; DNA immobilization; SAMs; rhodamine B
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Surface-enhanced Raman scattering (SERS) spectroscopy was used to monitor DNA hybridization of a fragment of the BRCA1 breast cancer susceptibility gene on modified silver surfaces. Rhodamine B was covalently attached to a 5'-amino-labeled oligonucleotide sequence (23 mer) through a succinimidyl ester intermediate in methanol. The silver surfaces were prepared by depositing a discontinuous layer (9.0 nm) of silver onto glass slides, which had been etched with HF to form a microwell platform, and subsequently modified with a monolayer of mercaptoundecanoic acid. The complementary probe was covalently attached to the silver surfaces using a succinimidyl ester intermediate in acetonitrile. The silver island substrate allows a very large enhancement of the Raman signal of the DNA-Rhodamine B, and clear distinction between hybridized samples and controls on a microwell array sampling platform. (C) 2002 Elsevier Science B.V All rights reserved.
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