Journal
JOURNAL OF CELL BIOLOGY
Volume 158, Issue 7, Pages 1171-1181Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200205102
Keywords
centrosome; centrioles; microtubules; microtubule-organizing centers; cell cycle
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Funding
- NIGMS NIH HHS [GM59363, R37 GM040198, GM030758, R37 GM040198-19, R01 GM059363-04, R01 GM059363, GM40198, R01 GM040198, R01 GM030758] Funding Source: Medline
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The centrosome usually replicates in a semiconservative fashion, i.e., new centrioles form in association with preexisting maternal centrioles. De novo formation of centrioles has been reported for a few highly specialized cell types but it has not been seen in vertebrate somatic cells. We find that when centrosomes are completely destroyed by laser microsurgery in CHO cells arrested in S phase by hydroxyurea, new centrosomes form by de novo assembly. Formation of new centrosomes occurs in two steps: similar to5-8 h after ablation, clouds of pericentriolar material (PCM) containing gamma-tubulin and pericentrin appear in the cell. By 24 h, centrioles have formed inside of already well-developed PCM clouds. This de novo pathway leads to the formation of a random number of centrioles (2-14 per cell). Although clouds of PCM consistently form even when microtubules are completely disassembled by nocodazole, the centrioles are not assembled under these conditions.
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