Journal
MOLECULAR CELL
Volume 10, Issue 4, Pages 895-905Publisher
CELL PRESS
DOI: 10.1016/S1097-2765(02)00690-1
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Funding
- NCI NIH HHS [T32 CA80416, CA88942] Funding Source: Medline
- NIGMS NIH HHS [GM49857, GM59224] Funding Source: Medline
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We have generated an artificial highly specific endonuclease by fusing domains of homing endonucleases I-Dmol and I-Crel and creating a new 1400 Angstrom(2) protein interface between these domains. Protein engineering was accomplished by combining computational redesign and an in vivo protein-folding screen. The resulting enzyme, E-Drel (Engineered I-Dmol/I-Crel), binds a long chimeric DNA target site with nanomolar affinity, cleaving it precisely at a rate equivalent to its natural parents. The structure of an E-Drel/DNA complex demonstrates the accuracy of the protein interface redesign algorithm and reveals how catalytic function is maintained during the creation of the new endonuclease. These results indicate that it may be possible to generate novel highly specific DNA binding proteins from homing endonucleases.
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