Journal
JOURNAL OF CLINICAL MICROBIOLOGY
Volume 40, Issue 10, Pages 3793-3797Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.40.10.3793-3797.2002
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During 1999, we used partial 16S rRNA gene sequencing for the prospective identification of atypical nonfermenting gram-negative bacilli isolated from patients attending our cystic fibrosis center. Of 1,093 isolates of nonfermenting gram-negative bacilli recovered from 148 patients, 46 (4.2%) gave problematic results with conventional phenotypic tests. These 46 isolates were genotypically identified as Pseudomonas aeruginosa (19 isolates, 12 patients), Achromobacter xylosoxidans (10 isolates, 8 patients), Stenotrophomonas maltophilia (9 isolates, 9 patients), Burkholderia cepacia genomovar I/III (3 isolates, 3 patients), Burkholderia vietnamiensis (I isolate), Burkholderia gladioli (I isolate), and Ralstonia mannitolilytica (3 isolates, 2 patients), a recently recognized species.
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