Journal
STRUCTURE
Volume 10, Issue 10, Pages 1317-1328Publisher
CELL PRESS
DOI: 10.1016/S0969-2126(02)00827-4
Keywords
tubulin; microtubules; electron crystallography; protein structure; dynamic instability
Funding
- NIGMS NIH HHS [GM35433, GM40633, GM26357] Funding Source: Medline
Ask authors/readers for more resources
We have obtained a 3D reconstruction of intact microtubules, using cryoelectron microscopy and image processing, at a resolution of about 8 Angstrom, sufficient to resolve much of the secondary structure. The level of detail in the map allows docking of the tubulin structure previously determined by electron crystallography, with very strong constraints, providing several important insights not previously available through docking tubulin into lower-resolution maps. This work provides an improved picture of the interactions between adjacent protofilaments, which are responsible for microtubule stability, and also suggests that some structural features are different in microtubules from those in the zinc sheets with which the tubulin structure was determined.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available