Journal
JOURNAL OF NEUROIMMUNOLOGY
Volume 131, Issue 1-2, Pages 98-103Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0165-5728(02)00256-4
Keywords
3-D model; Rhesus macaque; activation markers
Categories
Funding
- NCRR NIH HHS [RR00168, P51 RR000164, RR00164, P51 RR000168, K26 RR000168] Funding Source: Medline
- NIMH NIH HHS [R01 MH061192, MH61192] Funding Source: Medline
- NINDS NIH HHS [NS37654, R01 NS030769, R01 NS040237, R01 NS037654, NS40237, NS30769, NS35732] Funding Source: Medline
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The blood-brain barrier (BBB) has been modeled in vitro in a number of species, including rat, cow and human. Coculture of multiple cell types is required for the correct expression of tight junction proteins by microvascular brain endothelial cells (MBEC). Markers of inflammation, especially MHC-II, and cell adhesion molecules, such as VCAM-1, are not expressed on the luminal surface of the barrier under resting conditions. The thesus macaque model has been used to study early events of HIV-neuropathogenesis in vivo, but a suitable in vitro model has not been available for detailed mechanistic studies. Here we describe an in vitro thesus macaque blood-brain barrier that utilizes autologous MBEC and astrocytes. We believe that this model is highly relevant for examining immunological events at the blood-rain barrier and demonstrate its potential usefulness for examining early events in AIDS neuropathogenesis. (C) 2002 Elsevier Science B.V. All rights reserved.
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