Journal
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
Volume 50, Issue 10, Pages 1421-1424Publisher
HISTOCHEMICAL SOC INC
DOI: 10.1177/002215540205001015
Keywords
beta-galactosidase; X-gal staining; fixative; temperature
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Funding
- NCI NIH HHS [N01-CO-12400] Funding Source: Medline
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beta-Galactosidase (beta-Gal) staining is widely used to demonstrate specific gene expression during evaluation of gene targets in vivo. This technique is extremely sensitive to fixation. Optimal fixation conditions are necessary to obtain the maximal P-Gal activity. In this experiment, Carnoy's and three different aldehyde fixatives were used at different temperatures and over different time points. Kidneys from LacZ-stop-human alkaline phosphatase (ZA/P) double reporter mice were used to generate positive material for the experiment. The results show that glutaraldehyde combinative solution (LacZ) produced the most consistent and reliable results. Paraformaldehyde and formaldehyde were effective as fixatives only at 4C for a period of less than 4 hr, and Carnoy's solution destroyed P-Gal activity.
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