Journal
JOURNAL OF APPLIED MICROBIOLOGY
Volume 118, Issue 3, Pages 753-763Publisher
WILEY
DOI: 10.1111/jam.12743
Keywords
anti-virulence; morin hydrate; Staphylococcus aureus; virulence factor; -hemolysin
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Funding
- National Basic Research Program of China [2013CB127205]
- Research Fund for the Doctoral Program of Higher Education of China [20120061110079]
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AimsTo investigate the mechanism by which morin hydrate inhibits the haemolytic activity of -hemolysin (Hla), a channel-forming toxin that is important for the pathogenesis of disease in experimental animals, and its therapeutic effect against Staphylococcus aureus pneumonia in a mouse model. Methods and ResultsThe results from the in vitro (haemolysis, western blot and cytotoxicity assays) and in vivo (mouse model of intranasal lung infection) experiments indicated that morin hydrate, a natural compound with little anti-Staph.aureus activity, could effectively antagonize the cytolytic activity of Hla, alleviate human lung cell injury, and protect against mortality of Staph.aureus pneumonia in a mouse model of infection. Molecular dynamics simulations, free energy calculations and mutagenesis assays were further employed to determine the catalytic mechanism of inhibition, which indicated that a direct binding of morin to the Stem' domain of Hla (residues I107 and T109) and the concomitant change in conformation led to the inhibition of the self-assembly of the heptameric transmembrane pore, thus inhibiting the biological activity of Hla for cell lysis. ConclusionsMorin inhibited Staph.aureus virulence via inhibiting the haemolytic activity of -hemolysin. Significance and Impact of the StudyThese findings suggested that morin is a promising candidate for the development of anti-virulence therapeutic agents for the treatment of Staph.aureus infections.
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