Journal
PHYTOCHEMISTRY
Volume 61, Issue 3, Pages 339-343Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0031-9422(02)00237-6
Keywords
Brassica rapa; Cruciferae; Corolla; UV absorption; UV-B; nectar guide; pollination; flavonoids; isorhamnetin 3,7-0-di-beta-D-glucopyranoside
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The corolla of Brassica rapa has an UV-absorbing zone in its center, known as the nectar guide for attracting pollinating insects. The pigment which plays the role of the nectar guide was isolated from the petals and identified to be isorhamnetin 3,7-O-di-beta-D-glucopyranoside on the basis of MS and NMR spectroscopic data. The D-, L-configurations of the sugar moieties were determined by the fluorometric HPLC method. In plants raised in open field, there was a 13-fold higher content of the compound in the basal parts of the petals compared with the apical parts. This difference in flavonoid content is presumed to contribute to the visual attractiveness of B. rapa flowers to insect pollinators. (C) 2002 Elsevier Science Ltd. All rights reserved.
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