Journal
TOXICOLOGY IN VITRO
Volume 16, Issue 5, Pages 549-556Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0887-2333(02)00055-3
Keywords
bisphenol A; bisphenol A sulfate; phenol sulfotransferase; MCF-7; estrogenic chemicals
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Bisphenol A, an endocrine-disrupting chemical, is widely used in many consumer products. We previously showed the sulfo-conjugation of bisphenol A catalyzed by a human thermostable phenol sulfotransferase, ST1A3. The estrogenic potency of bisphenol A sulfate was compared with that of bisphenol A by an E-screen assay using human breast cancer MCF-7 cells. An increase in the expression level of an estrogen-responsive pS2 gene was also examined using MCF-7 cells after exposure to bisphenol A and its sulfate for their estrogenicity. Bisphenol A sulfate did not exhibit estrogenic effects at 0.1 muM (E-screen assay) and 1 mM (pS2 gene expression) compared with bisphenol A, which exhibited the effects at 3 nm (E-screen assay) and 1 muM (pS2 gene expression), respectively. We have therefore evaluated major roles of cytosolic phenol sulfotransferase in the human liver. Bisphenol A sulfation in human liver cytosols was inhibited by more than 90% by p-nitrophenol and quercetin, a typical substrate and specific inhibitor of phenol sulfotransferase, respectively. These results indicated that the estrogenicity of bisphenol A was abolished through its sulfation catalyzed by a human hepatic thermostable phenol sulfotransferase. (C) 2002 Elsevier Science Ltd. All rights reserved.
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