Journal
CHEMOSPHERE
Volume 49, Issue 3, Pages 315-322Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0045-6535(02)00317-X
Keywords
polycyclic aromatic hydrocarbons; biotransformation; mollusk; PAHs; contaminant
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Two species of marine clam, Mya arenaria and Protothaca staminea, were exposed to pyrene and 1-hydroxypyrene in small glass aquaria. After 10 days of exposure the clams were sacrificed, and both clam tissue and seawater were assayed for pyrene metabolites by using HPLC, fluorescence spectroscopy, HPLC-ESI-MS, GC-MS and H-1-NMR spectrometry. 1-Pyrenol-1-hydrogensulfate (pyrene-1-sulfate) was identified as the major water soluble metabolite formed from both pyrene and 1-hydroxypyrene by both species of clam. 1-Hydroxypyrene was identified as a minor metabolite of pyrene, and pyrenediol-hydrogen sulfate was identified as a minor metabolite of 1-hydroxypyrene. (C) 2002 Elsevier Science Ltd. All rights reserved.
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