Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 99, Issue 20, Pages 12669-12674Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.192463199
Keywords
bacteria; signal transduction; cyan fluorescent protein; yellow fluorescent protein
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In Escherichia coli chemotaxis, signaling depends on modulation of the level of phosphorylation of CheY, a small protein that couples receptors and flagellar motors. Working in vivo, we used fluorescence resonance energy transfer (FRET) to measure the interaction of CheYsimilar toP with its target, FIN. Binding of CheYsimilar toP to FIN was found to be much less cooperative than motor switching; however, under the conditions of our experiment, most of the FliM appeared to be in the cytoplasm. We studied signal processing times in the chemotaxis pathway by measuring the changes in CheYsimilar toP binding to FIN on flash release of caged chemoeffectors. Following sudden addition of attractant, the amount of CheYsimilar toP bound to FIN decayed exponentially with a rate constant of about 2 s(-1). Following sudden addition of repellent, FliM occupancy increased with a rate constant of about 20 s-1. Using these data, we were able to construct a simple model for the chemotactic pathway and to estimate values of rate constants for several key reactions.
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