Phytochrome in cotyledons regulates the expression of genes in the hypocotyl through auxin-dependent and -independent pathways

To elucidate the mechanism of plant responses to shading, we identified three promoter/enhancer trap lines (M812, J53, J59) that exhibited reporter expression in the hypocotyl in response to the end-of-day far-red light treatment. Interestingly, we found auxin-responsive genes in the vicinities of the reporter insertion sites in M812 and J53. We examined the effects of auxin on the reporter expression in these lines together with a previously identified N35 line. The results indicated that the reporter expression was induced by exogenous auxin in N35 and J53. Furthermore, an auxin transport inhibitor inhibited the responses of these lines to the end-of-day far-red light treatment, suggesting the involvement of auxin in the responses of plants to shading. By contrast, neither auxin nor the transport inhibitor affected the response in M812 and J59. Interestingly, J59 responded to ABA. Hence, ABA might be involved in the response as well. Analysis of the photoreceptive sites for the responses revealed the cotyledons, not the hypocotyl, are the major photoreceptive sites both in the auxin-responsive and ABA-responsive lines. Hence, some signals appeared to be transmitted from the cotyledons to the hypocotyl.