Journal
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
Volume 58, Issue -, Pages 1588-1592Publisher
BLACKWELL MUNKSGAARD
DOI: 10.1107/S0907444902014452
Keywords
protein crystallization; ferritin; concentration pulse technique; nucleation; structureless substrates
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The nucleation of horse spleen ferritin (HSF) crystals on substrates was investigated using a new modification of the double pulse technique. The influence of three different structureless 1 substrates (glass, glass covered by methyl groups and poly-L-lysin template) on the nucleation was studied. The boundaries in the phase-diagram, which separate zones of crystal nucleation and growth were obtained by keeping pH = 5.0, and using CdSO4 as crystallizing agent. The steady-state nucleation rates were determined. The energy required for critical nuclei formation was evaluated (10(-13) erg) and the sizes of critical nuclei were found (5 and 2 molecules).
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