Journal
MICROSCOPY AND MICROANALYSIS
Volume 8, Issue 5, Pages 375-391Publisher
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S1431927602010346
Keywords
cell death; confocal microscopy; immunohistological techniques; mitochondria; programmed cell death
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Funding
- NEI NIH HHS [R01 EY008886-10] Funding Source: Medline
- NHLBI NIH HHS [HL65314] Funding Source: Medline
- NINDS NIH HHS [NS39469] Funding Source: Medline
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Programmed cell death or apoptosis occurs in many tissues during normal development and in the normal homeostasis of adult tissues. Apoptosis also plays a significant role in abnormal development and disease. Increased interest in apoptosis and cell death in general has resulted in the development of new techniques and the revival of old ones. Each assay has its advantages and disadvantages that can render it appropriate and useful for one application, but inappropriate or difficult to use in another. Understanding the strengths and limitations of the assays would allow investigators to select the best methods for their needs.
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