Journal
BIOTECHNOLOGY AND BIOENGINEERING
Volume 80, Issue 1, Pages 33-41Publisher
JOHN WILEY & SONS INC
DOI: 10.1002/bit.10346
Keywords
biocatalysis; stereoselective epoxidation; styrene monooxygenase; pilot plant; Escherichia coli; integrated process; in situ product recovery; S-styrene oxide
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Recombinant Escherichia coli JM101(pSPZ10) cells produce the styrene monooxygenase of Pseudomonas sp. strain VLB120, which catalyzes the oxidation of styrene to (S)-styrene oxide at an enantiomeric excess larger than 99%. This biocatalyst was used to produce 388 g of styrene oxide in a two-liquid phase 30-L fed-batch bioconversion. The average overall volumetric activity was 170 U per liter over a period of more than 10 h, equivalent to mass transfer rates of 10.2 mmoles per liter per hour at a phase ratio of 0.5. At this transfer rate, the biotransformation system appeared to be substrate mass-transfer limited. The reactor had an estimated power input in the order of 5 W.L-1, which is close to values typically obtained with commercially operating units. The product could be easily purified by fractional distillation to a purity in excess of 97%. The process illustrates the feasibility of recombinant whole cell biotransformations in two-liquid phase systems with toxic substrates and products. (C) 2002 Wiley Periodicals, Inc.
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