Journal
FEBS LETTERS
Volume 529, Issue 2-3, Pages 309-312Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(02)03389-6
Keywords
iron metabolism; iron regulatory protein 1; iron-responsive element; ferritin; transferrin receptor
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Ethyl-3,4-dihydroxybenzoate (EDHB) is commonly utilized as a substrate analog and competitive inhibitor of prolyl 4-hydroxylases. These iron-dependent enzymes have received a lot of attention for their involvement in crucial biochemical pathways such as collagen maturation and oxygen sensing. Since EDHB is also capable of chelating the enzyme-bound iron, we study here its function as a chelator. We show that the affinity of EDHB for ferric iron is significantly lower than that of desferrioxamine. Nevertheless, EDHB is sufficient to promote effective iron deficiency in cells, reflected in the activation of the iron-responsive element/iron regulatory protein regulatory network. Thus, treatment of B6 fibroblasts with EDHB results in slow activation of iron regulatory protein 1 accompanied by an increase in transferrin receptor levels and reduction of the ferritin pool. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
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