Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 298, Issue 1, Pages 41-45Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/S0006-291X(02)02389-6
Keywords
ABC-family; estradiol-beta-glucuronide; organic anion; ABCA8
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We examined the transport capacity in Xenopus laevis oocytes of human EST KIAA0822/ABCAB, a member of the ABC superfamily. Substrates of ABCC2/MRP-2 such as [C-14]estradiol-beta-glucuronide, taurocholate, and LTC4, and of organic anion transporter (OAT), such as para-aminohippuric acid, ochratoxin-A, were significantly accumulated while tetraethylammonium and doxorubicin were not. The transport of [C-14]estradiol-beta-glucuronide was ATP-dependent and K-m and V-max values of 30.4 muM and 66.9 pmol/h/egg, respectively, were estimated. The transport of [C-14]estradiol-beta-glucuronide was inhibited by substrates/inhibitors of ABCC2/MRP-2, but not by those of the organic cation transporter and multidrug resistance protein (MDR)-1. KIAA0822/ABCA8 possesses two ATP-binding sites and fourteen transmembrane domains. Northern blot analysis revealed expression in most organs, especially in heart, skeletal muscle, and liver. Thus, ABCA8 is a new member of the xenobiotic transporter ABC-subfamily. (C) 2002 Elsevier Science (USA). All rights reserved.
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