Journal
IMMUNOLOGY LETTERS
Volume 84, Issue 1, Pages 69-76Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0165-2478(02)00145-1
Keywords
thymus; rat; adrenergic nerve fibres; mast cells; quantitative analysis of images; 6-OH dopamine
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The interactions between adrenergic nerve fibres and mast cells (MCs) were studied in the thymus of adult and old rats by morphological methods and by quantitative analysis of images (QAIs). The whole thymus was drawn in adult (12 months old) rats: normal, sympathectomized or electrostimulated. Thymuses from the above-mentioned animals were weighed, measured and dissected. Thymic slices were stained with eosin orange for detection of microanatomical details and with Bodian's method for identification of the whole nerve fibres. Thymic MCs were stained with Astrablau. Histofluorescence microscopy was used for staining of adrenergic nerve fibres. Finally, all morphological results were submitted to the QAIs and statistical analysis of data. Our results suggest that after surgical sympathectomy, the greater part of adrenergic nerve fibres disappear while related MCs appear to show less evident fluorescence and few granules. On the contrary, electrostimulation of the cervical superior ganglion induced an increase in the fluorescence of adrenergic nerve fibres and of related MCs. (C) 2002 Elsevier Science B.V. All rights reserved.
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