Transmissibility of field isolates of soybean viruses by Aphis glycines

During the 2001 growing season, 191 symptomatic soybean (Glycine max (L.) Merr.) plants were dug from production plots in Indiana, Wisconsin, and Kentucky. Alfalfa mosaic virus(AMV), Bean pod mottle virus (BPMV), Bean yellow mosaic virus (BYMV), Peanut stunt virus (PSV), Tobacco ringspot virus (TRSV), and Soybean mosaic virus (SMV) were identified: No mixed infections were observed. The ability of the soybean aphid (Aphis glycines Matsamura) to transmit field isolates of these viruses was tested. Using naturally infected field- or greenhouse-grown soybean plants as sources, six isolates of SMV and two isolates of AMV were transmitted using a short feeding assay. One of two isolates of TRSV was transmitted by A. glycines in one of four experiments using an extended feeding transmission assay. BPMV was not transmitted by A. glycines in assays involving 11 field isolates: and over 840 aphids. One field isolate each of BYMV and PSV were tested and no transmission by A. glycines was observed.