Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 975, Issue 2, Pages 229-243Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0021-9673(99)01250-9
Keywords
reviews; retention characteristics; stationary phases, LC; carbon sorbents; porous graphitized carbon
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Reversed-phase separations provides a versatile technique in high-performance liquid chromatography. Porous graphitized carbon (PGC) support shows unique retention characteristics. Separations on PGC columns use typical reversed-phase eluents (water and organic modifiers miscible with water), however, the retention order of solutes generally does not follow their hydrophobicity order. Molecular hydrophobicity influences but not determines the elution order of any set of solutes. The properties of these supports, mechanisms of retention and application are discussed, along with correlations which can guide the choice of solvent combinations for typical separations. (C) 2002 Elsevier Science B.V. All rights reserved.
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