Journal
BIOTECHNOLOGY LETTERS
Volume 24, Issue 21, Pages 1807-1813Publisher
SPRINGER
DOI: 10.1023/A:1020633909762
Keywords
anthracycline; DNA intercalation; gel mobility shift; Streptomyces
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An improved, rapid and inexpensive gel mobility shift assay was developed for the screening of anthracycline antibiotics. The assay based on the intercalation activity of these molecules into dsDNA was used to assess the activity of partially purified antibiotics. Detection limits were of 0.1 ng ml(-1) with an average run time of 2 h. The assay is potentially useful for high throughput screening in bioprospecting, for monitoring fermentation production phases and downstream purification process.
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