Journal
BIOLOGICAL TRACE ELEMENT RESEARCH
Volume 89, Issue 2, Pages 165-175Publisher
HUMANA PRESS INC
DOI: 10.1385/BTER:89:2:165
Keywords
selenium; glutathione peroxidase; thioredoxin reductase; gene expression; enzyme activity
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Selenium (Se) is an essential trace element in animals and human, however, excess Se intake can result in adverse health effects. Se supplementation increased glutathione peroxidase (GSH-Px) and thioredoxin reductase (TR) expressions in Se-deficient rats. However, little information is available on the relationship between Se overexposure on GSH-Px and TR mRNA levels and activities. In this study, the effects of Se overexposure on GSH-Px and TR mRNA levels and activities were investigated by reverse transcription-polymerase chain reaction (RT-PCR) and activity assay. Experimental groups of male Wistar rats were injected intraperitoneally (ip) with sodium selenite at doses of 20, 40, and 80 mug Se/kg/d for 15 d, respectively. Se levels were elevated dose-dependently in rat liver, kidney, and testis tissues. GSH-Px mRNA levels and activities in the liver and testis for rats injected with 20 mug Se/kg/d were increased significantly as compared with those in the control group. However, intraperitoneal injection of 40 and 80 mug Se/kg/d dramatically decreased GSH-Px mRNA expression and activity in liver and testis. The changes of TR mRNA level and activity in the liver and kidney were similar to those of GSH-Px in the liver and testis when supplemented with 40 and 80 mug Se/kg/d. There were no significant effects of Se status on kidney GSH-Px and testis TR expressions. The results suggested that Se overexposure had an adverse effect on GSH-Px and TR mRNA levels and activities, and there could be tissue differences in the regulation of selenoprotein levels.
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